Proteomics and Cell Culture

In the AG Mailänder, we run a cell culture lab for general cell culture purposes at a genetic safety level S1. Standard equipment like laminar air flows, incubators, microscopes, liquid nitrogen cell storage etc. are available and can be used in collaboration with the group.

Besides this we are equipped with a label-free, quantitative proteomics setup consisting of an ultra performance liquid chromatography (UPLC) coupled to a state-of-the-art mass spectrometer, a Waters Synapt G2Si. We run the system with a nanoESI ionization source and use a determined proteomics software to automatically evaluate the data and determine the proteome of complex protein mixtures. This enabled us to determine and elucidate the protein corona of nanoparticles in complex protein mixtures. This is highly interesting as the protein corona changes the fate of nanocarriers in cell cultures but also in living organisms. It is a key factor to determine the biological function of a nanocarrier system.