Accurate structural models of biological systems can be obtained by integrative approaches that properly combine multiple sources of information, such as experimental data and a priori physico-chemical knowledge. In this talk, I will give an overview of the methodological approaches that we have been developing as well as a series of applications to systems of outstanding biological importance. Specifically, I will focus on the determination of structural ensembles of intrinsically disordered systems using NMR data and on the use of cryo-electron microscopy data to unravel the continuous dynamics of flexible parts of ordered systems. Finally, I will present an open-source, freely-available module of the PLUMED library (www.plumed.org), which enables the simultaneous determination of structure and dynamics of conformationally heterogeneous systems by integrating experimental data with a priori information.
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