All-Optical Sensing

Triplet–triplet annihilation upconversion performed in nanoconfinement is used as a minimally invasive, all-optical sensing tool to determine local temperature and oxygen concentration in the extracellular liquid microenvironment.

The all-optical sensing laboratory is equipped with a comprehensive suite of optical tools for full characterization of absorption and emission profiles, including lifetime and quantum yield, of the organic specimens under study, and is available to all group members.

Besides this we are equipped with table top incubator PECON P-Set 2000 with adjustable cell culture temperature control, engineered (fully tuneable CO₂ content) and optical quality window, allowing application of immersion objectives, with high numerical aperture (NA = 1). The information signal passes a high precision dual camera image splitter with freely tuneable transmission / reflection spectrum (W-VIEW Gemini-2C, Hamamatsu Photonics) combined with two gated Image Intensifier (C9016-26, Hamamatsu Photonics) and acquired by two ORCA-spark cameras (C11440-36U, Hamamatsu Photonics). The experimental setup is designed for simultaneous two-dimensional observation of the respiratory activity of a cell ensemble.